Prosecutors want to charge Mohammed Ali Malek, a 27-year-old Tunisian, with homicide and people-trafficking.
Mr Malek's lawyer says his client is keen to tell his story to prove his innocence to the court in Catania.
One of the 28 survivors has given nearly five hours of testimony. Three others are due to speak later.
Prosecutors say survivors identified Mr Malek as the captain of the boat who accidentally rammed the overloaded fishing boat into a merchant ship that had come to rescue it, last Sunday.
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