The striker went off on 59 minutes on Wednesday after a Diego Godin tackle.
He was compelled to miss Argentina's World Cup 2018 qualifier against Venezuela prior this month with a crotch damage, yet has played in each of the five La Liga amusements for his club this season.
The Argentine could return for Barca's Champions League tie with Manchester City on 19 October.
Preceding that tie, Messi will be discounted of recreations against Borussia Monchengladbach in the Champions League, Sporting Gijon and Celta Vigo in La Liga, and World Cup qualifiers against Peru and Paraguay.
Sergio Busquets was likewise constrained off with damage in the second 50% of Wednesday's amusement as Angel Correa's equalizer offset Ivan Rakitic's opener at the Nou Camp.
Barcelona tweeted not long after the amusement that Lionel Messi will be out for three weeks
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